![xi yunbo metcn xi yunbo metcn](https://www.asiansets.com/wp-content/uploads/2015/07/2011.11.01.jpg)
Joly, Philippe Falconnet, Pierre-Alain André, Janine Weill, Nicole Reyrolle, Monique Vandenesch, François Maurin, Max Etienne, Jerome Jarraud, Sophie Quantitative Real-Time Legionella PCR for Environmental Water Samples: Data Interpretation It is thus concluded that dd PCR technology can be applied for routine quantification of GMOs, or any other domain where quantitative analysis of food and feed samples is needed. Finally, dd PCR throughput and cost are advantageous relative to those of q PCR for routine GMO quantification. Moreover, when compared to q PCR, the dd PCR assay showed better repeatability at low target concentrations and a greater tolerance to inhibitors. It offers a dynamic range over four orders of magnitude, greater than that of cd PCR. The sensitivity (five target DNA copies) of the dd PCR assay compares well with those of individual q PCR assays and of the chamber digital PCR (cd PCR) approach. The dd PCR system is shown to offer precise absolute and relative quantification of targets, without the need for calibration curves. Key performance parameters of the assay were determined. Using dd PCR duplex assay, we have measured the absolute numbers of MON810 transgene and hmg maize reference gene copies in DNA samples.
![xi yunbo metcn xi yunbo metcn](https://img.tiai.cc/MetCN/fhtmvl02ni1.jpg)
However, its use is limited for detecting and quantifying very small numbers of DNA targets, as in some complex food and feed matrices. Real-time quantitative polymerase chain reaction (q PCR) is currently used for quantitative molecular analysis of the presence of GMOs in products. In this study, the applicability of droplet digital PCR (dd PCR) for routine analysis in food and feed samples was demonstrated with the quantification of genetically modified organisms (GMOs). Morisset, Dany Štebih, Dejan Milavec, Mojca Gruden, Kristina Žel, Jana
![xi yunbo metcn xi yunbo metcn](https://img.tiai.cc/MetCN/umcbf0415xi.jpg)
Quantitative Analysis of Food and Feed Samples with Droplet Digital PCR